کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1367632 | 981641 | 2006 | 8 صفحه PDF | دانلود رایگان |
Phosphorothioate oligonucleotides manufactured by standard phosphoramidite techniques using 2′-deoxyadenosine- or 2′-O-(2-methoxyethyl)-5-methylcytosine-loaded solid supports contain branched impurities consisting of two chains linked through the exocyclic amino group of the 3′-terminal nucleoside of one chain and the 3′-terminal hydroxyl group of another via a P(O)SH group. These impurities are not produced when a universal, non-nucleoside derivatized support is used.
Phosphorothioate oligonucleotides manufactured by standard phosphoramidite techniques using nucleoside-loaded supports contain high molecular weight impurities of the type shown. These impurities are avoided if a novel universal solid support is used.Figure optionsDownload as PowerPoint slide
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 16, Issue 3, 1 February 2006, Pages 607–614