کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1369197 | 981764 | 2013 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Base recognition of gap sites in DNA–DNA and DNA–RNA duplexes by short oligonucleotides
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Base recognition of gap sites in DNA–DNA and DNA–RNA duplexes by short oligonucleotides Base recognition of gap sites in DNA–DNA and DNA–RNA duplexes by short oligonucleotides](/preview/png/1369197.png)
چکیده انگلیسی
To increase base recognition capability and sensitivity, we propose the separation of a commonly used single-probe system for oligonucleotide analysis into a set of three probes: a fluorophore-labeled probe, a promoter probe, and a short probe. In this study, we found that the probes of only 4 nt in length can selectively bind the corresponding gap site on complexes consisting of the target, fluorophore-labeled probe, and promoter probe, exhibiting a more than 14-fold difference in ligation between the matched and mismatched sequences. Moreover, we demonstrated that the immobilized short probes accurately recognized the sequences of the gap sites.
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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 23, Issue 11, 1 June 2013, Pages 3448–3451
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 23, Issue 11, 1 June 2013, Pages 3448–3451
نویسندگان
Ken Yamada, Akihiro Ohkubo, Yousuke Esaka, Takashi Kanamori, Yoshiaki Masaki, Kohji Seio, Mitsuo Sekine,