کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1373457 981898 2007 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
A high-throughput assay for O-GlcNAc transferase detects primary sequence preferences in peptide substrates
چکیده انگلیسی

O-GlcNAc transferase (OGT) catalyzes the addition of N-acetylglucosamine (O-GlcNAc) onto a diverse array of intracellular proteins. Although hundreds of proteins are known to be modified by O-GlcNAc, a strict amino acid consensus sequence for OGT has not been identified. In this study, we describe the development of a high-throughput assay for OGT and use it to profile the specificity of the enzyme among a panel of peptide substrates.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 17, Issue 14, 15 July 2007, Pages 3851–3854
نویسندگان
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