کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1375754 981944 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Use of γ-aminopropyl-coated glass surface for the patterning of oligonucleotides through oxime bond formation
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Use of γ-aminopropyl-coated glass surface for the patterning of oligonucleotides through oxime bond formation
چکیده انگلیسی

The present work reports on the preparation of glass surfaces coated with NPPOC-protected aminooxy groups and their use for the patterning of oligonucleotides on glass slides and in capillary tubes. The method involves the use of surfaces coated with amino groups using (γ-aminopropyl)triethoxy silane and subsequent grafting of the aminooxy groups by using the activated ester 1. The NPPOC-protected aminooxy groups on the surfaces can be cleaved upon irradiation. The free aminooxy groups so obtained are subsequently reacted with aldehyde-containing oligonucleotides to achieve efficient surface patterning.

The patterning of oligonucleotides on glass slides and into the inner wall of capillary tubes have been achieved by using oxime bond formation.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 18, Issue 8, 15 April 2008, Pages 2540–2543
نویسندگان
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