Novel mitochondria-localizing TEMPO derivative for measurement of cellular oxidative stress in mitochondria

Neurodegenerative disorders, such as Alzheimer’s and Parkinson’s diseases, and apoptosis, are thought to be associated with oxidative stress affecting mitochondria. In this study, we designed and synthesized a fluorescein-tagged TEMPO derivative, compound 1, with triphenylphosphino moiety. Synthesized 1 localized in mitochondria and detected oxidative stress in an endotoxic model of a mouse macrophage-like cell line. Compound 1 is therefore a potentially useful probe for evaluating oxidative stress in mitochondria.

We designed and synthesized a mitochondria-localizing TEMPO derivative, 1, and demonstrated that synthesized probe 1 localized and detected oxidative stress in mitochondria in an activated mouse macrophage-like cell line.Figure optionsDownload as PowerPoint slide