Layer-by-layer polysaccharide-coated liposomes for sustained delivery of epidermal growth factor

• DODAB liposomes were coated with the xanthan and galactomannan biopolymers by LbL.
• The non-ionic interaction between xanthan and galactomannan stabilize the LbL.
• Stable LbL coating of liposomes extend the release of EGF up to 5 times.

A three-dimensional layer-by-layer (LbL) structure composed by xanthan and galactomannan biopolymers over dioctadecyldimethylammonium bromide (DODAB) liposome template was proposed and characterized for protein drug delivery. The polymers and the surfactant interaction were sufficiently strong to create a LbL structure up to 8 layers, evaluated using quartz crystal microbalance (QCM) and zeta potential analysis. The polymer–liposome binding enthalpy was determined by isothermal titration calorimetry (ITC). The bilayer of biopolymer-coated liposomes with diameters of 165 (±15) nm, measured by dynamic light scattering (DLS), and ζ-potential of −4 (±13) mV. These bilayer-coated nanoparticles increased up to 5 times the sustained release of epidermal growth factor (EGF) at a first order rate of 0.005 min−1. This system could be useful for improving the release profile of low-stability drugs like EGF.