کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1383764 982360 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dipsacus asperoides polysaccharide induces apoptosis in osteosarcoma cells by modulating the PI3K/Akt pathway
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Dipsacus asperoides polysaccharide induces apoptosis in osteosarcoma cells by modulating the PI3K/Akt pathway
چکیده انگلیسی


• A polysaccharide (ADAPW) was isolated from the alkaline extraction of D. asperoides roots.
• ADAPW inhibited the proliferation of HOS cells by inducing apoptosis.
• ADAPW caused a loss of mitochondrial membrane potential.
• ADAPW stimulated a generation of reactive oxygen species (ROS).
• ADAPW suppressed the protein expression of PI3K and pAkt.

An alkaline extractable and water-soluble polysaccharide (ADAPW), with an average molecular weight of 16 kDa, was purified from the alkaline extraction of the roots of Dipsacus asperoides. Monosaccharide component analysis indicated that ADAPW was composed of glucose, rhamnose, arabinose and mannose in a molar ratio of 8.54:1.83:1.04:0.42. This study aimed to investigate the effect of ADAPW on the viability of human osteosarcoma cell line HOS cells, and explore the possible mechanisms. The results revealed that ADAPW inhibited the proliferation of HOS cells in a dose-dependent manner by inducing apoptosis. Furthermore, treatment with ADAPW caused a loss of mitochondrial membrane potential and accumulation of reactive oxygen species (ROS). In addition, Western blot analysis demonstrated that ADAPW down-regulated the protein expressions of PI3K and phosphorylated Akt (pAkt) in HOS cells. Taken together, induction of apoptosis on HOS cells by ADAPW was mainly associated with ROS production, mitochondrial dysfunction, and inhibition of PI3K/Akt signaling pathway. So this finding suggests that ADAPW may be potentially effective in cancer prevention against human osteosarcoma.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 95, Issue 2, 20 June 2013, Pages 780–784
نویسندگان
, , , , , , , , ,