کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1383809 1500873 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison of lipopolysaccharide structures of Bordetella pertussis clinical isolates from pre- and post-vaccine era
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Comparison of lipopolysaccharide structures of Bordetella pertussis clinical isolates from pre- and post-vaccine era
چکیده انگلیسی


• We compare the LPS structures of five Bordetella pertussis strains and human isolates.
• A molecular model showing the conformation of LPS main molecular species is displayed.
• The data demonstrate the conservation of LPS structures before and after the vaccine era.

Endotoxins are lipopolysaccharides (LPS), and major constituents of the outer membrane of Gram-negative bacteria. Bordetella pertussis LPS were the only major antigens, of this agent of whooping-cough, that were not yet analyzed on isolates from the pre- and post-vaccination era. We compared here the LPS structures of four clinical isolates with that of the vaccine strain BP 1414. All physico-chemical analyses, including SDS–PAGE, TLC, and different MALDI mass spectrometry approaches were convergent. They helped demonstrating that, on the contrary to some other B. pertussis major antigens, no modification occurred in the dodecasaccharide core structure, as well as in the whole LPS molecules. These results are rendering these major antigens good potential vaccine components. Molecular modeling of this conserved LPS structure also confirmed the conclusions of previous experiments leading to the production of anti-LPS monoclonal antibodies and defining the main epitopes of these major antigens.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 378, 30 August 2013, Pages 56–62
نویسندگان
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