کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1383815 1500873 2013 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The unique structure of complete lipopolysaccharide isolated from semi-rough Plesiomonas shigelloides O37 (strain CNCTC 39/89) containing (2S)-O-(4-oxopentanoic acid)-α-d-Glcp (α-d-Lenose)
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
The unique structure of complete lipopolysaccharide isolated from semi-rough Plesiomonas shigelloides O37 (strain CNCTC 39/89) containing (2S)-O-(4-oxopentanoic acid)-α-d-Glcp (α-d-Lenose)
چکیده انگلیسی


• The complete structure of semi-rough LPS of P. shigelloides O37 has been established.
• The presence of the novel sugar α-d-Lenose residue was identified.
• The α-d-Lenose has not been found in nature so far.

The complete structure of semi-rough lipopolysaccharide (SR-LPS) of Plesiomonas shigelloides CNCTC 39/89 (serotype O37) has been investigated by 1H and 13C NMR spectroscopy, matrix-assisted laser-desorption/ionization time-of-flight MS, and chemical methods. The following structure of the single unit of the O-antigen has been established: Figure optionsDownload as PowerPoint slidein which α-d-Lenp is (2S)-O-(4-oxopentanoic acid)-α-d-Glcp residue which has not been found in nature. The absolute configuration of oxopentanoic acid moiety in α-d-Lenose residue was determined by NOESY experiment combined with molecular modeling (MM2 force field). The decasaccharide core is substituted at C-4 of the β-d-Glcp residue with a single pentasaccharide unit. Lipid A is built of a β-d-GlcpN4P-(1→6)-α-d-GlcpN1P disaccharide asymmetrically substituted with fatty acids. It was concluded that the core oligosaccharide and the lipid A are identical with those in P. shigelloides CNCTC 113/92 Niedziela et al. (2002) 9 and Lukasiewicz et al. (2006).10

Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 378, 30 August 2013, Pages 98–107
نویسندگان
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