کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1383908 982380 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cytotoxic and antioxidant effects of unsaturated hyaluronic acid oligomers
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Cytotoxic and antioxidant effects of unsaturated hyaluronic acid oligomers
چکیده انگلیسی

Hyaluronate lyase cleaves hyaluronan chains at a β-d-GalNAc-(1→4)-β-d-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-β-d-gluc-4-enuronosyl)-N-acetyl-d-glucosamine. In the present paper, the recombinant hyaluronate lyase (EC 4.2.2.1, HA lyase) of Streptococcus pyogenes bacteriophage H4489A was produced, purified and applied to digest hyaluronan producing unsaturated hyaluronan oligomers. The resulted unsaturated hyaluronan oligomers were purified and subjected to anion-exchange high performance chromatography. The cytotoxic activities of naturally hyaluronan, hyaluronate lyase and unsaturated hyaluronan oligomers were tested and evaluated against HEp-2 (human laryngeal carcinoma), Daoy (human medulloblastoma), MCF-7 (human breast adenocarcinoma), and WiDr (human colon adenocarcinoma) tumor cell lines. Also, their antioxidant activities have been examined by the inhibition of lipid oxidation and free radical scavenging assays.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 82, Issue 4, 11 November 2010, Pages 1116–1123
نویسندگان
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