کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1384132 | 982390 | 2009 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Establishment of CTAB Turbidimetric method to determine hyaluronic acid content in fermentation broth
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The characters of visible spectra on the formation of turbidity between hyaluronic acid (HA) and cetyltrimethylammonium bromide (CTAB) were investigated. The effects of reaction time and temperature on the formation of turbidity were also explored. Consequently, CTAB Turbidimetric method (CTM), a quick and safe method to determine HA content in large batch fermentation broth samples, was established. CTM and Bitter–Muir method (BMM) were respectively applied to measure HA content and were thoroughly compared. The results indicate that CTM was superior to BMM in terms of accuracy, precision and sensitivity. CTM is a promising alternative to BMM to determine HA content in fermentation broth.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Polymers - Volume 78, Issue 1, 4 August 2009, Pages 178–181
Journal: Carbohydrate Polymers - Volume 78, Issue 1, 4 August 2009, Pages 178–181
نویسندگان
Yong-Hao Chen, Qiang Wang,