کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1384375 | 982401 | 2013 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Characterization of mucilage polysaccharides, arabinogalactanproteins and cell-wall hemicellulosic polysaccharides isolated from flax seed meal: A wealth of structural moieties Characterization of mucilage polysaccharides, arabinogalactanproteins and cell-wall hemicellulosic polysaccharides isolated from flax seed meal: A wealth of structural moieties](/preview/png/1384375.png)
The present study aimed at analyzing the structural features of seed mucilage and cell-wall polysaccharides which accounted for 41% of the mass of flax meal (FM). A combination of high molar-mass mucilage-like polysaccharides (rhamnogalacturonan and arabinoxylan) was released from FM in water, together with arabinogalactan proteins and glucans. About half of FM homogalacturonans was extracted using a calcium chelator and boiling water. Hemicellulosic xyloglucans and xylans were further extracted with 1 M KOH, in ∼13% FM-sugars yield. Structural characterization of the xyloglucan using specific enzyme hydrolysis, ion exchange chromatography (HPAEC) and matrix assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectroscopy showed the presence of XXXG type xyloglucan, but also that of XXGG-structure, possibly characteristic of flax seeds. Hydrolysis of xylans with endo-(1 → 4)-β-d-xylanase, and analysis of the neutral and acidic oligosaccharides by MALDI-TOF-MS showed that xylan consisted of β-(1 → 4)-linked-d-xylopyranose backbone with some zones (DP 5–7) substituted with 4-O-MeGlcA\GlcA\Glc residues.
► Thirty percent of the matter of flax meal can be selected for specific applications.
► Besides RG-I and AX mucilages, HGA, Ara and Glc rich moieties, and AGP are present in flax meal.
► Structure of flax seed hemicelluloses was proposed for the first time.
► Structural analysis confirms that the isolated xylan was a branched heteroxylan.
► The xyloglucan is XXXG-type but also contain XXGG structure.
Journal: Carbohydrate Polymers - Volume 93, Issue 2, 2 April 2013, Pages 651–660