کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1385609 982452 2006 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Glycan analysis of recombinant Aspergillus niger endo-polygalacturonase A
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Glycan analysis of recombinant Aspergillus niger endo-polygalacturonase A
چکیده انگلیسی
The enzyme endo-polygalacturonase A, or PGA, is produced by the fungus, Aspergillus niger, and appears to play a critical role during invasion of plant cell walls. The enzyme has been homologously overexpressed in order to provide sufficient quantities of purified enzyme for structural and biological studies. We have characterized this enzyme in terms of its post-translational modifications (PTMs) and found it to be both N- and O-glycosylated. Additionally, we have characterized the glycosyl moieties using MALDI-TOF and LC-ESI mass spectrometry. The characterization of all PTMs on PGA, along with molecular modeling, allows us to reveal potential roles played by the glycans in modulating the interaction of the enzyme with other macromolecules.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 341, Issue 14, 16 October 2006, Pages 2370-2378
نویسندگان
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