Technology optimization for polysaccharides (POP) extraction from the fruiting bodies of Pleurotus ostreatus by Box–Behnken statistical design

Response surface methodology (RSM), based on A Box–Behnken design (BBD), was used to optimize the extraction conditions of polysaccharides (POP) from the fruiting bodies of Pleurotus ostreatus. Four independent variables such as extraction temperature (°C), ratio of water to raw material, number of extraction and extraction time (h) were investigated. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods. The optimum extraction conditions, determined by the 3-D response surface and contour plots derived from the mathematical models, were as follows: extraction temperature 94.9 °C, ratio of water to raw material 22, number of extraction 4, and extraction time 2.7 h. Under these conditions, the experimental value was 64.1 ± 1.42, which is well matched with value predicted by the model.