کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1386917 | 982540 | 2011 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effect of the degree of oxidation on the physicochemical and biological properties of Grifola frondosa polysaccharides Effect of the degree of oxidation on the physicochemical and biological properties of Grifola frondosa polysaccharides](/preview/png/1386917.png)
Polysaccharide extracted from Grifola frondosa was subjected to 2,2,6,6-tetramethyl-1-piperidine oxoammonium ion (TEMPO)-mediated oxidation of which effects on the structural and biological properties of the polysaccharide were investigated as a function of degree of oxidation. Successive oxidation of the polysaccharide was confirmed by 13C NMR spectroscopy and the molecular weight change of the oxidized polysaccharide was observed, decreasing from 10.6 × 105 Da to 7.5 × 105 Da by 100% oxidation. The oxidation also caused an increase in the water solubility of the polysaccharide while its viscosity was significantly reduced. In addition, when human fibrosarcoma HT1080 cells were treated with 100% oxidized polysaccharide, their in vitro growth was effectively inhibited. However, the oxidation reduced the activity of superoxide dismutase up to 82% at a concentration of 2.5 mg/mL. Thus, the chemical modification by TEMPO-mediated oxidation was shown to play a significant role in the biological properties of the polysaccharides derived from Grifola frondosa.
Journal: Carbohydrate Polymers - Volume 83, Issue 3, 30 January 2011, Pages 1298–1302