کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1387199 | 982555 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Optimization of extraction of Tremella fuciformis polysaccharides and its antioxidant and antitumour activities in vitro Optimization of extraction of Tremella fuciformis polysaccharides and its antioxidant and antitumour activities in vitro](/preview/png/1387199.png)
Optimization of extraction of Tremella fuciformis polysaccharides was investigated using response surface methodology in this paper. T. fuciformis polysaccharides were extracted using boiling water at different extraction temperatures (80–100 °C), times (3.5–4.5 h) and ratios of solvent to raw material (4–6). The effect of extraction conditions on the yield of T. fuciformis polysaccharides were studied using a three-level three-factor Box–Behnken design. The results showed that the highest yield (3.08%) of T. fuciformis polysaccharides was reached at extraction temperature 100 °C, extraction time 4.5 h and ratio of solvent to raw material 5 (v/v). In addition, T. fuciformis polysaccharides could scavenge superoxide anion and hydroxyl radicals. At last, it could still be found that antitumour activities of T. fuciformis polysaccharides increased from 73.4% to 92.1% with increasing concentration of polysaccharides. Pharmacology experiment indicated that T. fuciformis polysaccharides was useful to the therapy of free radical injury and cancer diseases.
Journal: Carbohydrate Polymers - Volume 81, Issue 2, 11 June 2010, Pages 420–424