Structural characterization and gastroprotective property of a novel glucofructan from Allium ampeloprasum var. porrum

• A polysaccharide was isolated from Allium ampeloprasum var. porrum.
• The chemical structure was established by spectroscopic techniques and chemical conversions.
• The gastroprotective property was investigated using in vivo experimental models.

A new polysaccharide with an estimated weight-average molar mass of 2.6 × 103 was isolated from Allium ampeloprasum var. porrum by hot water extraction, and purified by Sephacryl S-300 HR high-resolution chromatography. It was composed of d-fructose and d-glucose in 10:6 molar ratio, respectively. The structure of the glucofructan was investigated by chemical and spectroscopic methods, including methylation analysis, nuclear magnetic resonance, and electrospray mass spectrometry (ES-MS). The results permitted the structure of the glucofructan to be written as α-d-Glcp-(1→1)-β-d-Fruf-(2→1)-{[α-d-Glcp-(1→6)-β-d-Fruf-(2→6)]-β-d-Fruf-(2→1)}4-β-d-Fruf-(2↔1)-α-d-Glcp. Results of the present study indicated that this new glucofructan exhibited significant gastroprotective property, using in vivo experimental models.

α-d-Glcp-(1→1)-β-d-Fruf-(2→1)-{[α-d-Glcp-(1→6)-β-d-Fruf-(2→6)]-β-d-Fruf-(2→1)}4-β-d-Fruf-(2↔1)-α-d-Glcp.Figure optionsDownload as PowerPoint slide