کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1387969 982748 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural analysis of a highly sulfated fucan from the brown alga Laminaria cichorioides by tandem MALDI and ESI mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Structural analysis of a highly sulfated fucan from the brown alga Laminaria cichorioides by tandem MALDI and ESI mass spectrometry
چکیده انگلیسی

Water-soluble polysaccharide fractions were extracted from the brown alga Laminaria cichorioides. Samples were collected monthly from May to October in Troitsa Bay (Japan Sea, Russia). Analysis showed that the content and monosaccharide composition of the fractions changed with the collection season. Fucoidan was isolated and purified from the most fucose-rich fraction, collected in July, and subjected to autohydrolysis to obtain fucooligosaccharides, suitable for mass-spectrometric analysis. Both ESIMS and MALDI-TOFMS analyses show that multisulfated (up to 3) fucooligosaccharides with polymerization degree n from 2 to 5, including mono- and disulfated-fucose residues, were the major products of autohydrolysis. The structural features of the fucooligosaccharides and their alditol derivatives were elucidated by tandem MALDI-TOFMS and ESIMS. The results obtained allowed us to conclude that fragments of the fucoidan, collected in July, were predominantly linked with a (1→3)-type of linkage and that sulfate groups occupied mostly C-2 or C-2/C-4 of the α-l-fucose residues.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 345, Issue 15, 13 October 2010, Pages 2206–2212
نویسندگان
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