کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1387975 982748 2010 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural characterization of glycoprotein NGAL, an early predictive biomarker for acute kidney injury
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Structural characterization of glycoprotein NGAL, an early predictive biomarker for acute kidney injury
چکیده انگلیسی

Neutrophil gelatinase-associated lipocalin (NGAL) is a promising new renal biomarker that can reduce the time to diagnose acute kidney injury (AKI). There is little information available about complex glycans on NGAL. Detailed structural characterization of NGAL is necessary to understand the structural variability of NGAL used as a standard in the NGAL immunoassay. This study demonstrated that 7–9% of mutant (C87S) recombinant NGAL was N-glycosylated and no O-glycosylation was detected. The NGAL sequence was confirmed by nanoLC/MS/MS following in gel and in solution trypsin digestion, and the N-glycosylation site was localized by MS/MS. Six different mutant recombinant NGAL samples (samples A–F) were analyzed in this study; however, these samples demonstrated two different glycan patterns. Forty-one N-glycans were detected in sample A and the more abundant N-glycans were unsialylated. Forty-three N-glycans were detected in sample F and the more abundant N-glycans were sialylated. Each of the other four samples (B–E) had a similar N-glycan pattern as sample F.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 345, Issue 15, 13 October 2010, Pages 2252–2261
نویسندگان
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