کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1388463 | 982795 | 2011 | 7 صفحه PDF | دانلود رایگان |
α-Cyclosophoro-octadecaoses (α-C18), produced by Rhodobacter sphaeroides, are mostly homogeneous in size with 18 glucose units per ring as the predominant form. α-C18s are linked by β-(1→4)-linkages and one α-(1→6)-linkage and are also known to be highly substituted by acetyl (0–2 per mol) and/or succinoyl groups (1–7 per mol). We isolated and purified α-C18 and successfully used it in capillary electrophoresis (CE) as a chiral additive for the separation of five flavanones and flavanone-7-O-glycosides, including naringenin, hesperetin, eriodictyol, homoeriodictyol, isosakuranetin, and hesperidin. Throughout the CE experiment with unsubstituted α-C18 (uα-C18) obtained after alkaline treatment of the isolated α-C18, we found that successful chiral separation critically depends on the presence of succinate substituents attached to α-C18 in CE, suggesting that succinoylation of α-C18 is decisive for effective stereoisomeric separation.
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Journal: Carbohydrate Research - Volume 346, Issue 1, 3 January 2011, Pages 133–139