Mass spectrometric characterization of a two-glycosyltransferase tandem reaction for assembly of tetrasaccharide repeating unit of Escherichia coli O77 O-antigen

- MS provides valuable information about in vitro characterization of glycosyltransferases.
- MS is highly valuable for precise structure elucidation of lipooligosaccharides.
- The wbaD gene and wbaC gene from E. coli O77 encoding mannosyltransferases were characterized.
- This study leads to complete characterization of O77 O-repeating unit synthetic pathway.