Structural studies of O-polysaccharide isolated from Cronobacter sakazakii Sequence Type 12 from a case of neonatal necrotizing enterocolitis

• Cronobacter sakazakii NTU 696 causes necrotising enterocolitis.
• O-polysaccharide was isolated from C. sakazakii NTU 696 Sequence Type 12.
• Chemical structure of OPS was determined using NMR spectroscopy and chemical methods.

The O-polysaccharide (OPS) of Cronobacter sakazakii NTU 696 (Sequence Type 12) from a case of neonatal necrotizing enterocolitis was isolated from the polysaccharide fraction obtained after lipopolysaccharide (LPS) hydrolysis. Purified OPS was analyzed by NMR spectroscopy (1H, COSY, TOCSY, NOESY, HSQC, HSQC-TOCSY and HMBC experiments) and chemical methods. Obtained monosaccharide derivatives analyzed by gas chromatography and gas chromatography-mass spectrometry allowed the identification of six sugar components.Performed experiments enabled to establish a structure of the OPS repeating unit of C. sakazakii NTU 696, as:

Figure optionsDownload as PowerPoint slide