A heteropolysaccharide from an edible hybrid mushroom pfle 1p: structural and immunostimulating studies

• A water soluble heteropolysaccharide was isolated from hybrid mushroom pfle 1p.
• The polysaccharide contained glucose, galactose, and mannose in a ratio of 4:2:1.
• Its structure was established by chemical and 1D/2D NMR analyses.
• It showed macrophage, splenocyte, and thymocyte activation.

A water-soluble heteropolysaccharide (PS-I) having molecular weight ∼2.1 × 105 Da was isolated from hot aqueous extract of the fruit bodies of hybrid mushroom pfle 1p. The hybrid mushroom pfle 1p was obtained through intergenic protoplast fusion between Pleurotus florida and Lentinula edodes. The heteropolysaccharide contained d-glucose, d-galactose, and d-mannose in a molar ratio of nearly 4:2:1. The structural investigation of PS-I has been carried out using sugar and methylation analyses as well as 1D/2D NMR experiments (1H, 13C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HSQC, and HMBC). Based on the results of these experiments, the repeating unit of the PS-I was established as:Figure optionsDownload as PowerPoint slidePS-I showed in vitro macrophage activation by NO production and also stimulated splenocytes and thymocytes.

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