Isolation, purification and structural characterization of an acetylated heteroglycan from the unripe fruits of Manilkara zapota L.

A water soluble polysaccharide isolated from the hot water extract of the unripe fruits of Manilkara zapota L. was found to consist of 3-O-acyl-l-rhamnose, l-arabinose, 3-O-acetyl-d-methyl galacturonate in a molar proportion of nearly 1:1:1. Structural investigation of the polysaccharide was carried out using total hydrolysis, methylation analysis; periodate oxidation followed by GLC–MS, and NMR experiments. On the basis of the above experiments it is concluded that the following repeating unit is present in the polysaccharide.Figure optionsDownload as PowerPoint slide

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► An acetylated heteroglycan was isolated from unripe fruits of Manilkara zapota L.
► Linkage analysis was carried out using total hydrolysis and methylation analysis.
► Complete structure was assigned using 2D NMR spectrum.