کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1390562 983102 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
TEMPO-mediated glycoconjugation: a scheme for the controlled synthesis of polysaccharide conjugates
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
TEMPO-mediated glycoconjugation: a scheme for the controlled synthesis of polysaccharide conjugates
چکیده انگلیسی

A method for the controlled conjugation of polysaccharides (PSs) to carrier proteins based on the stoichiometric oxidation of PSs with 2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPO) is described. In this scheme, a PS can be stoichiometrically activated, by the formation of minimal amounts of carboxylic acids with TEMPO, and subsequently coupled to a carrier protein through amide bond formation. Our exploratory studies included a number of readily available glucans, which were oxidized with several combinations of TEMPO-sodium hypochlorite preparations followed by conjugation to BSA. Following these investigations, more structurally complex bacterial capsular PSs were stoichiometrically oxidized with TEMPO-sodium hypochlorite and conjugated to BSA. Hypochlorite was deemed to be the reaction component responsible for the extent of oxidation. Collectively, the data showed this approach to be effective in designing PS-conjugates with no disruption to the structural integrity and immunochemical properties of the PS, and thus has the potential to become a reliable method for glycoconjugate vaccine synthesis.

Lane 1, Precision Plus protein markers (Bio-Rad); Lane 2, BSA Standard; Lane 3, BSA-Nigeran; Lane 4, BSA-Corn starch; Lane 5, BSA-CPSActinobacillus; Lane 6, BSA-CPSCampylobacter.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 346, Issue 2, 1 February 2011, Pages 343–347
نویسندگان
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