کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1390859 983152 2007 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A simple method of preparing diverse neoagaro-oligosaccharides with β-agarase
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
A simple method of preparing diverse neoagaro-oligosaccharides with β-agarase
چکیده انگلیسی

In order to prepare pure and well-defined oligosaccharides from agarose in a rapid and simple manner, an enzymatic degradation method was developed, which includes degradation with either recombinant β-agarase (EC 3.2.1.81) AgaA or AgaB and gel permeation chromatography. Agarose was degraded with AgaA at the optimized conditions, yielding 47% and 45% of neoagarotetraose and neoagarohexaose, respectively. These neoagaro-oligosaccharides were conveniently separated by consecutive column chromatography on Bio-Gel P2 or P6 and were identified by FACE. The structure of these neoagaro-oligosaccharides was confirmed by MALDI-TOF MS and 13C NMR spectroscopy.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Carbohydrate Research - Volume 342, Issue 8, 11 June 2007, Pages 1030–1033
نویسندگان
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