Fungal cell wall polysaccharides isolated from Discula destructiva spp.

The alkali-extractable water-soluble polysaccharides F1SS isolated from the cell wall of four species of Discula destructiva have been studied by methylation analysis and NMR spectroscopy, and their idealized structures established as where n ≈ 2 for strains CBS 109771 and CBS 133.91, n ≈ 1 for CBS 132.91, and it has an intermediate value in strain CBS 130.91. The mannan core was obtained by mild hydrolysis of the F1SS polysaccharide and its structure consisted of a skeleton of α-(1→6)-mannopyranan, with around one out of eleven residues substituted at C-2 by short chains (one to six units) of 2-substituted mannopyranoses.

The idealized repeating unit of the polysaccharide isolated from Discula destructiva is proposed to beFigure optionsDownload as PowerPoint slide