کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1391783 | 983641 | 2006 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Evolution of High-Branching Deoxyribozymes from a Catalytic DNA with a Three-Way Junction Evolution of High-Branching Deoxyribozymes from a Catalytic DNA with a Three-Way Junction](/preview/png/1391783.png)
SummaryHere, we report the evolution of two star-shaped (five-way junction) deoxyribozymes from a catalytic DNA containing a three-way junction scaffold. The transition was shown to be a switch rather than a gradual progression. The star-shaped motifs, surprisingly, only took five selection cycles to be detected, and another four to dominate the evolving population. Chemical probing experiments indicated that the two deoxyribozymes belong to the same family despite noticeable variations in both the primary sequence and the secondary structure. Our findings not only describe the evolution of high-branching nucleic acid structures from a low-branching catalytic module, but they also illustrate the idea of deriving a rare structural motif by sampling the sequence variants of a given functional nucleic acid.
Journal: - Volume 13, Issue 10, October 2006, Pages 1061–1069