کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1393947 984012 2009 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Putting Synthesis into Biology: A Viral View of Genetic Engineering through De Novo Gene and Genome Synthesis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Putting Synthesis into Biology: A Viral View of Genetic Engineering through De Novo Gene and Genome Synthesis
چکیده انگلیسی

SummaryThe rapid improvements in DNA synthesis technology hold the potential to revolutionize biosciences in the near future. Traditional genetic engineering methods are template dependent and make extensive but laborious use of site-directed mutagenesis to explore the impact of small variations on an existing sequence “theme.” De novo gene and genome synthesis frees the investigator from the restrictions of the pre-existing template and allows for the rational design of any conceivable new sequence theme.Viruses, being among the simplest replicating entities, have been at the forefront of the advancing biosciences since the dawn of molecular biology. Viral genomes, especially those of RNA viruses, are relatively short, often less than 10,000 bases long, making them amenable to whole genome synthesis with the currently available technology. For this reason viruses are once again poised to lead the way in the budding field of synthetic biology—for better or worse.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 16, Issue 3, 27 March 2009, Pages 337–347
نویسندگان
, , ,