Structural characteristics of thermosensitive chitosan glutamate hydrogels in variety of physiological environments

• Properties of thermosensitive chitosan glutamate are presented (FTIR, WAXS).
• Determined changes in hydrogel after conditioning in water, buffer at pH 7 and pH 2.
• On the basis of structure changes in water a mechanism formation was proposed.

In this paper the properties of thermosensitive chitosan hydrogels prepared with the use of chitosan glutamate and β-glycerophosphate are presented. The study is focused on the determination of changes in the hydrogel structure in different environments: during conditioning in water and buffer at pH 7 and pH 2 respectively. The structure of gels was observed under the Scanning Electron Microscopy (SEM) and was investigated by infrared (IR) spectroscopy. The crystallinity of gel structure was determined by X-ray diffraction analysis (XRD). On the basis of structural changes during the conditioning in water a mechanism of their formation was proposed.