The role of preorganization of hydrazone moieties on tetrathiacalix[4]arene platform for their conformational and binding properties from the view of structural investigation

► The IR, NMR data, conformational and dynamic behaviour of tetrahydrazone thiacalix[4]arenes. ► The X-ray crystal structures of cone and 1,3-alternate tetrahydrazone thiacalix[4]arenes. ► The influence of medium, calixarene platform, substituents on conformational properties. ► The correlation between extraction efficiency data and the conformational features of calix[4]arene hydrazones.