کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1403952 984857 2006 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel cationic polyaspartamide with covalently linked carboxypropyl-trimethyl ammonium chloride as a candidate vector for gene delivery
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Novel cationic polyaspartamide with covalently linked carboxypropyl-trimethyl ammonium chloride as a candidate vector for gene delivery
چکیده انگلیسی

The non-viral gene vector properties of a protein-like polymer, the α,β-poly(N-2-hydroxyethyl)-d,l-aspartamide (PHEA) were investigated after its derivatization with 3-(carboxypropyl)trimethyl-ammonium chloride (CPTA) as molecule bearing cationic groups, in order to obtain stable polycations able to condense DNA. PHEA was firstly functionalized with hydrazide pendant groups by reaction with hydrazine monohydrate (HYD), obtaining the polyhydrazide α,β-poly(N-2-hydroxyethyl/carbazate)-d,l-aspartamide (PHEA–HYD). In this paper we reported that polymer functionalization degree can be easily modulated by varying reaction conditions, so allowing us to produce two PHEA derivatives at different molar percentage of hydrazide groups. Subsequently, condensation reaction of PHEA–HYD copolymers with CPTA yielded α,β-poly(N-2-hydroxyethyl)-N-carbazate[N′-(3-trimethylammonium chloride)propylhydrazide]-d,l-aspartamide (PHEA–HYD–CPTA) polycation derivatives. In vitro studies were carried out to evaluate polycations ability to complex DNA and to protect it from nuclease degradation. Obtained results demonstrated the good efficiency of our new PHEA-polycations derivatives, PHEA–HYD–CPTA, to complex and condense genomic material even at very low polycation/DNA weight ratio.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Polymer Journal - Volume 42, Issue 4, April 2006, Pages 823–834
نویسندگان
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