Study of the inclusion interaction of HP-γ-cyclodextrin with bupropion and its analytical application

• The inclusion complex between BUP and HP-γ-CD was studied by spectroscopy.
• Formation of this 1:1 inclusion complex in aqueous phase has been confirmed by Job’s method of continuous variation.
• The structure of the complex was investigated by FTIR and NMR analyses.
• A spectrophotometric method was established to the determination of BUP in pharmaceuticals.

Inclusion complex formation between bupropion (BUP) and hydroxypropyl-γ-cyclodextrin (HP-γ-CD) was studied by Fourier transformation infrared (FT-IR), nuclear magnetic resonance (NMR) and UV spectroscopy. The main factors affecting inclusion interaction were discussed in detail. The inclusion complex of bupropion and hydroxypropyl-γ-cyclodextrin was studied at pH 5 in aqueous phase. Stoichiometry of the complex was found to be 1:1 and apparent formation constant (log K) was determined as 3.54 ± 0.01, suggesting a tendency of the drug to enter HP-γ-CD cavity. All obtained information proved the formation of BUP/HP-γ-CD inclusion complex. A significant enhancement of absorption intensity of bupropion in presence of HP-γ-CD was shown. Due to the property a sensitive spectrophotometric method was elaborated for determination of active substance in bulk solution. At optimum experimental conditions, a linear relationship between absorbance and concentration of bupropion is observed in range of 4–60 μg mL−1 with limit detection of 0.32 μg mL−1 and correlation coefficient of 0.9991. The proposed method was applied successfully to the determination of BUP in pharmaceutical preparations and the results were satisfactory in comparison to official method.

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