کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
14072 1072 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Atomic tritium as an instrument for study of protein behavior at the air–water interface
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Atomic tritium as an instrument for study of protein behavior at the air–water interface
چکیده انگلیسی

Atomic tritium was successfully applied as an instrument for study of protein behavior at the air–water interface. Samples of lysozyme solution in 20 mM phosphate buffer (pH 7.0) with concentration of 2 mg/ml incubated at the room temperature for 1 h were exposed to bombardment with tritium atoms generated on hot tungsten wire in special vacuum device. This procedure resulted in substitution of hydrogen atoms by radioactive tritium in the thin surface layer of studied preparations. Analysis of experimental data on intramolecular radioactivity distribution in lysozyme and computer simulation of tritium bombardment allowed us to suggest two equally probable opposite orientations of lysozyme molecule in the adsorption layer at the air–water interface.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomolecular Engineering - Volume 24, Issue 1, February 2007, Pages 125–129
نویسندگان
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