A novel method for the determination of fast green in grape wine based on resonance Rayleigh scattering

• A novel method detecting fast green (FCF) was proposed.
• The developed method was successfully used to detect FCF in grape wine.
• The developed method has multi-readout.

A novel resonance Rayleigh scattering method was developed for the determination of fast green (FCF) in grape wine. In pH 2.5 Britton Robinson (BR) buffer solution, the scattering signal of acridine orange (AO) was remarkably enhanced after adding trace amount of FCF and forming an ion-association complex, which not only resulted in the change of absorption spectrum, fluorescence spectra, but also led to a significant enhancement of resonance Rayleigh scattering (RRS), frequency doubling scattering (FDS), and second order scattering (SOS). The linear ranges and detection limits for RRS, SOS and FDS were 2–45 × 10−6 mol L−1, 2–24 × 10−6 mol L−1, 2–20 × 10−6 mol L−1, and 8.0 × 10−8 mol L−1, 4.7 × 10−7 mol L−3, 1.0 × 10−7 mol L−3, respectively. In this work, the optimum conditions, the influencing factors and the effects of coexisting substances on the reaction were investigated. The method can be applied to the determination of FCF in grape wine and the results were satisfactory.

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