کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
14082 1144 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effect of different growth factors on human osteoblasts activities: A possible application in bone regeneration for tissue engineering
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Effect of different growth factors on human osteoblasts activities: A possible application in bone regeneration for tissue engineering
چکیده انگلیسی

Cultured human primary osteoblasts reproduce the phenotypic differentiation and maturation of cells in vivo. We have investigated the influence of three isoforms of transforming growth factor beta (TGF-β1, TGF-β2 and TGF-β3), three fibroblast growth factors (FGF-2, FGF-4 and FGF-6) and the active metabolite of Vitamin D [1,25-(OH)2D3] on proliferation, alkaline phosphatase activity and mineralization of human osteoblasts during a period of 24 days of culture. TGF-β isoforms and three FGFs examined have been proved to be inducers of osteoblasts proliferation (higher extent for TGF-β and FGF-2) and inhibitors of alkaline phosphatase activity and osteoblasts mineralization. Combination of these growth factors with the active form of Vitamin D induced osteodifferentiation. In fact Vitamin D showed an additive effect on alkaline phosphatase activity and calcium content, induced by FGF-2 and TGF-β in human osteoblast. These results highlight the potential of proliferating cytokines’ combination with mineralizing agents for in vitro bone growth induction in bone tissue engineering.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomolecular Engineering - Volume 24, Issue 6, December 2007, Pages 613–618
نویسندگان
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