Effect of a glucose-triazole-hydrogenated cardanol conjugate on lipid bilayer membrane organization and thermotropic phase transition

• GTHCC perturbs lipid bilayer membrane organization.
• Below 30 mol% of GTHCC in the aqueous media , it decreases the Tm of DPPC membrane.
• Above 30 mol%, GTHCC and DPPC MLVs co-solubilize to form mixed lipid complex aggregates.

This work focuses on the membrane perturbation, solubilisation and thermotropic phase transition process of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) MLVs induced by a glucose-triazole-hydrogenated cardanol conjugate (GTHCC). GTHCC is a recently introduced non toxic sugar derivative. Differential scanning calorimetry (DSC) and fluorescence molecular probe based techniques have been used to understand the concentration dependent membrane perturbation, solubilisation and thermotropic phase transition process of DPPC MLVs. The phase transition temperature of DPPC MLVs decreases with increase in mol% of GTHCC. At higher concentration above 10 mol%, GTHCC was significantly perturbed the membrane organization. The intrinsic fluorescence of GTHCC is also found to be sensitive towards phase behaviour and changes in membrane organization of DPPC MLVs.

New glucose-triazole-hydrogenated cardanol conjugate (GTHCC): interactions with lipid membrane.Figure optionsDownload as PowerPoint slide