Investigation of 3D silvernanodendrite@glass as surface-enhanced Raman scattering substrate for the detection of Sildenafil and GSH

A solid-phase dendritic Ag nanostructure was synthesized in the presence of silk fibroin biomacromolecule and planted on the glass to form three-dimensional (3D) silvernanodendrite@glass film. When NO3-, Cl− and SO42- were added in the synthesis process of the film to study their influence on the Raman activity of this substrate using MB as probe molecule, it’s found that the substrate with Cl−1 gives the most intensive enhancement, and two ways were proposed to explain this phenomenon. Its superiority in practical application of surface-enhanced Raman scattering (SERS) was verified by analyzing the characteristic Raman spectrum of Sildenafil between 1150 cm−1 and 1699 cm−1. Besides, the absorption mechanism of GSH on the film through the role of peptide bond was analyzed. GSH interacts strongly with the silver surface via the ν(CS) in two different conformers. The carboxyl and the amide groups are also involved in the adsorption process. In this experiment, we synthesized, studied and applied this as-growth substrate and found some information about its interaction with different molecular bonds and functional groups of peptide.

► A new three-dimensional (3D) silvernanodendrite@glass film was synthesized.
► The influence of ions on the Raman activity of this film was analyzed.
► The superiority of the film in practical application of SERS was verified.
► The absorption mechanism of GSH on the film through the peptide bond was analyzed.