Spectral characteristics of fluorescence and circular dichroism of aflatoxin B1 reaction with its anti-idiotypic antibody

► F(ab′)2 fragment was obtained by pepsin digestion of rabbit anti-AFB1 antibody (Ab1). ► Ab2 was generated upon the immunization of mice with F(ab′)2 fragment. ► The characterization of Ab2 was investigated by icELISA. ► The fluorescence spectroscopy and circular dichroism spectrum were employed to study interaction among F(ab′)2, AFB1 and Ab2.