کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1410600 | 1501873 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Equilibrium and kinetic analysis on the folding of hen egg lysozyme in the aqueous-glycerol solution
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Equilibrium and kinetic analysis on the folding of hen egg lysozyme in the aqueous-glycerol solution Equilibrium and kinetic analysis on the folding of hen egg lysozyme in the aqueous-glycerol solution](/preview/png/1410600.png)
چکیده انگلیسی
In the present study, the equilibrium and kinetic analysis on the folding of hen egg lysozyme in the aqueous-glycerol solutions have been reported by means of fluorescence and circular dichroism spectra. Addition of glycerol increases the stability of lysozyme on the guanidine-induced denaturation, and the unfolding transition of lysozyme is from a two-state into a three-state mechanism. On the kinetic experiments, the folding pathway of lysozyme has been changed in the presence of glycerol. An overshot phenomenon in fluorescence spectra appears and the preformed ellipticity within the dead time is weakened. These results reveal that a new intermediate state with a non-native hydrophobic core but lack of the stable secondary structure is populated in the folding pathway of lysozyme, and the conversion of it to the native state is decelerated due to the excess of non-native hydrophobic interactions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Structure - Volume 891, Issues 1â3, 26 November 2008, Pages 167-172
Journal: Journal of Molecular Structure - Volume 891, Issues 1â3, 26 November 2008, Pages 167-172
نویسندگان
Ling-zhi Wu, Bao-liang Ma, Yue-biao Sheng, Wei Wang,