کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1410711 | 1501893 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Study of the effect of Cal-Red on the secondary structure of human serum albumin by spectroscopic techniques
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Study of the effect of Cal-Red on the secondary structure of human serum albumin by spectroscopic techniques Study of the effect of Cal-Red on the secondary structure of human serum albumin by spectroscopic techniques](/preview/png/1410711.png)
چکیده انگلیسی
The effect of Cal-Red on the structure of human serum albumin (HSA) was studied using Resonance light scattering (RLS), Fourier transformed Infrared (FT-IR) and Circular dichroism (CD) spectroscopic methods. The RLS spectroscopic results show that the RLS intensity of HSA was significantly increased in the presence of Cal-Red. The binding parameters of HSA with Cal-Red were studied at different temperatures of 289, 299, 309 and 319 K at pH 4.1. It is indicated by the Scatchard plots that the binding constant K decreased from 4.03 Ã 108 to 7.59 Ã 107 l/mol and the maximum binding number N decreased from 215 to 152 with increasing the temperature, respectively. The binding process was exothermic and spontaneous, as indicated by the thermodynamic analyses, and the major part of the binding energy is hydrophobic interaction. The enthalpy change ÎH0, the free energy change ÎG0 and the entropy change ÎS0 of 289 K were calculated to be â42.75 kJ/mol, â47.56 kJ/mol and 16.66 J/mol K, respectively. The alterations of protein secondary structure in the presence of Cal-Red in aqueous solution were quantitatively calculated from FT-IR and CD spectroscopy with reductions of α-helices content about 5%, β-turn from 10% to 2% and with increases of β-sheet from 38% to 51%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Structure - Volume 846, Issues 1â3, 26 November 2007, Pages 112-118
Journal: Journal of Molecular Structure - Volume 846, Issues 1â3, 26 November 2007, Pages 112-118
نویسندگان
Lijun Dong, Xingguo Chen, Zhide Hu,