Structural study of an immunoenhancing polysaccharide isolated from an edible hybrid mushroom of Pleurotus florida and Lentinula edodes

The water-soluble polysaccharide isolated from hot aqueous extract of the fruit bodies of somatic hybrid mushroom (pfle 1q) obtained through intergenic protoplast fusion between Pleurotus florida and Lentinula edodes consists of d-galactose and d-mannose in a molar ratio of nearly 2:1. The polysaccharide showed immunoenhancing activity by stimulating the macrophage, splenocyte, and thymocyte. Based on the results of acid hydrolysis, methylation analysis, periodate oxidation, and NMR experiments (1H, 13C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HSQC and HMBC) the repeating unit of the purified polysaccharide was established as

► A water soluble mannogalactan was isolated from the hybrid mushroom Pfle 1q.
► The structure of the mannogalactan was established by chemical and 2D NMR analysis.
► This polysaccharide showed splenocyte, thymocyte and macrophage activation.