Separation and structural elucidation of a polysaccharide CC30w-1 from the fruiting body of Coprinus comatus

• Polysaccharide CC30w-1 was purified from the fruiting body of Coprinus comatus.
• CC30w-1 is composed of L-Fuc and D-Gal in the ratio of 1:3.84.
• The structure of CC30w-1 is elucidated by methylated analysis and the NMR spectroscopy.

In this study, a water soluble polysaccharide (CC30w-1), with a molecular mass of 1.03×104, was isolated from the fruiting body of Coprinus comatus. The polysaccharide moiety was composed of L-Fuc and D-Gal in the ratio of 1:3.84. The structure of CC30w-1was elucidated by methylated analysis, GC–MS, 1H and 13C NMR spectroscopy, together with 1H–1H COSY, TOCSY, HMQC and HMBC. Results showed that the polysaccharide consists of the following repeating units:α−L−Fucp1↓2→6)−α−D−Galp−(1→6)−α−D−Galp−(1→6)−α−D−Galp−(1→6)−α−D−Galp(1→