کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1423716 1509042 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Generation of a safe and effective live viral vaccine by virus self-attenuation using species-specific artificial microRNA
ترجمه فارسی عنوان
تولید یک واکسن ایمن و مؤثر زنده ویروسی با خودکفایی ویروس با استفاده از میکروسکوپ الکترونیتی مصنوعی خاص
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی

Vaccination with live attenuated vaccines (LAVs) is an effective way for prevention of infectious disease. While several methods are employed to create them, efficacy and safety are still a challenge. In this study, we evaluated the feasibility of creating a self-attenuated RNA virus expressing a functional species-specific artificial microRNA. Using influenza virus as a model, we produced an attenuated virus carrying a mammalian-specific miR-93 expression cassette that expresses a viral nucleoprotein (NP)-specific artificial microRNA from an insertion site within the non-structural (NS) gene segment. The resulting engineered live-attenuated influenza virus, PR8-amiR-93NP, produced mature and functional artificial microRNA against NP in mammalian cells, but not in avian cells. Furthermore, PR8-amiR-93NP was attenuated by 104 fold in mice compared with its wild-type counterpart. Importantly, intranasal immunization with PR8-amiR-93NP conferred cross-protective immunity against heterologous influenza virus strains. In short, this method provides a safe and effective platform for creation of live attenuated RNA viral vaccines.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 207, 10 June 2015, Pages 70–76
نویسندگان
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