کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1423720 1509042 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Combinatorial gene construct and non-viral delivery for anti-obesity in diet-induced obese mice
ترجمه فارسی عنوان
ساخت ژن ترکیبی و تحویل غیر ویروسی برای ضد چاقی در موش های چاق مبتلا به رژیم غذایی
کلمات کلیدی
چاقی، ژن ترکیبی درمانی، حامل ژن غیر ویروسی، لپتین، پلی اتیل اتیل آمیلوئید
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی

The combinatorial peptidergic therapy of islet amyloid polypeptide (IAPP) and leptin (LEP) analogues was once an optimistic option in treating obese animals and patients. However, the need for frequent administrations and its negative side effects prevent it from being a viable choice. Here, we developed a combinatorial gene therapy of IAPP and LEP, where two genes are inserted into a single plasmid with self-cleaving furin and 2A sites to treat diet-induced obese (DIO) mice. The developed plasmid DNA (pDNA) individually produced both IAPP and LEP peptides in vitro and in vivo. The pDNA was delivered with a non-viral polymeric carrier, and its once-a-week administrations demonstrated a synergistic loss of body weight and significant reductions of fat mass, blood glucose, and lipid levels in DIO mice. The results suggest that the combinatorial gene therapy would have higher potential than the peptidergic approach for future translation due to its improved practicability.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 207, 10 June 2015, Pages 154–162
نویسندگان
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