کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1424741 986735 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
HPMA-oligolysine copolymers for gene delivery: Optimization of peptide length and polymer molecular weight
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
HPMA-oligolysine copolymers for gene delivery: Optimization of peptide length and polymer molecular weight
چکیده انگلیسی

Polycations are one of the most frequently used classes of materials for non-viral gene transfer in vivo. Several studies have demonstrated a sensitive relationship between polymer structure and delivery activity. In this work, we used reverse addition-fragmentation chain transfer (RAFT) polymerization to build a panel of N-(2-hydroxypropyl)methacrylamide (HPMA)-oligolysine copolymers with varying peptide length and polymer molecular weight. The panel was screened for optimal DNA-binding, colloidal stability in salt, high transfection efficiency, and low cytotoxicity. Increasing polyplex stability in PBS correlated with increasing polymer molecular weight and decreasing peptide length. Copolymers containing K5 and K10 oligocations transfected cultured cells with significantly higher efficiencies than copolymers of K15. Four HPMA-oligolysine copolymers were identified that met the desired criteria. Polyplexes formed with these copolymers demonstrated both salt stability and transfection efficiencies on-par with poly(ethylenimine) PEI in cultured cells.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 155, Issue 2, 30 October 2011, Pages 303–311
نویسندگان
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