کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1426104 986794 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A modified protocol for efficient DNA encapsulation into pegylated immunoliposomes (PILs)
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
A modified protocol for efficient DNA encapsulation into pegylated immunoliposomes (PILs)
چکیده انگلیسی

Effective delivery of transgenes to the brain through a non-invasive route has great prospects for treating diseases in the central nervous system (CNS). Slightly anionic pegylated immunoliposomes (PILs) have been shown to be effective in reaching the CNS, but efficient DNA encapsulation into the liposomes used for this purpose is technically difficult and hard to reproduce. We here use an improved protocol for DNA encapsulation of pegylated immunoliposomes based on ethanol-mediated DNA condensation. We introduce a dialysis step following DNA encapsulation to remove ethanol and show that this step is necessary to ensure complete nucleolytic removal of non-encapsulated DNA. The uptake of the pegylated immunoliposomes into human cells was documented by live-cell confocal imaging, and specific targeting to the human insulin receptor was shown by inhibiting clathrin-mediated endocytosis.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 139, Issue 2, 15 October 2009, Pages 140–145
نویسندگان
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