کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1426474 986814 2008 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Polyhydroxyethylaspartamide-spermine copolymers: Efficient vectors for gene delivery
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
پیش نمایش صفحه اول مقاله
Polyhydroxyethylaspartamide-spermine copolymers: Efficient vectors for gene delivery
چکیده انگلیسی

Aim of this paper was that to prepare biocompatible, polyaspartamide based copolymers containing spermine or spermine/hydrophobic side chains able to condense nucleic acids and to transfect mammalian cells.Copolymers were prepared starting from α,β-poly-(N-2-hydroxyethyl)-d,l-aspartamide (PHEA) and exploiting the reactive hydroxyl groups in the polymeric side chains by subsequent activation reactions to obtain PHEA-Spermine (PHEA-Spm) and PHEA-Spermine-Butyramide (PHEA-Spm-C4). Molecular, physico-chemical and biological characterization of copolymers and interpolyelectrolyte complexes with plasmid DNA was performed.Experimental results evidenced that these copolymers are able to form complexes with plasmid DNA already at low polycation/DNA weight ratio ranging from 0.75/1 to 2/1. Interpolyelectrolyte complexes with decreased size were obtained when increasing the polycation/DNA weight ratio, until nanosized dimensions were reached. Copolymers as well as complexes were not haemolytic and non toxic in vitro. In vitro cell transfection with PHEA derivatives showed good biocompatibility and high transfection efficiency (luciferase) in cancer cells in comparison with commercially available, but toxic transfection agents.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Controlled Release - Volume 131, Issue 1, 6 October 2008, Pages 54–63
نویسندگان
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