کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1428790 1509182 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Graphene and carbon nanotube nanocomposite for gene transfection
ترجمه فارسی عنوان
نانوکامپوزیت نانولوله گرافن و کربن برای انتقال ژن
کلمات کلیدی
اکسید گرافن، انتقال ژن، نانولوله های کربنی، کامپوزیت، زنده ماندن سلولها، پلاسما
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


• Facile water dispersible GCN was prepared exfoliating MWCNTs by oxygen plasma etching.
• Oxygen content groups were attached on the GCN surfaces changing its hydrophobicity.
• Low concentrations of GCN showed low cytotoxicity for NIH-3T3 and NG97 line cells.
• The GCN and pIRES plasmid conjugated with the GFP gene.
• Gene transfection was studied using this new material.

Graphene and carbon nanotube nanocomposite (GCN) was synthesised and applied in gene transfection of pIRES plasmid conjugated with green fluorescent protein (GFP) in NIH-3T3 and NG97 cell lines. The tips of the multi-walled carbon nanotubes (MWCNTs) were exfoliated by oxygen plasma etching, which is also known to attach oxygen content groups on the MWCNT surfaces, changing their hydrophobicity. The nanocomposite was characterised by high resolution scanning electron microscopy; energy-dispersive X-ray, Fourier transform infrared and Raman spectroscopies, as well as zeta potential and particle size analyses using dynamic light scattering. BET adsorption isotherms showed the GCN to have an effective surface area of 38.5 m2/g. The GCN and pIRES plasmid conjugated with the GFP gene, forming π-stacking when dispersed in water by magnetic stirring, resulting in a helical wrap. The measured zeta potential confirmed that the plasmid was connected to the nanocomposite. The NIH-3T3 and NG97 cell lines could phagocytize this wrap. The gene transfection was characterised by fluorescent protein produced in the cells and pictured by fluorescent microscopy. Before application, we studied GCN cell viability in NIH-3T3 and NG97 line cells using both MTT and Neutral Red uptake assays. Our results suggest that GCN has moderate stability behaviour as colloid solution and has great potential as a gene carrier agent in non-viral based therapy, with low cytotoxicity and good transfection efficiency.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 39, 1 June 2014, Pages 288–298
نویسندگان
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