کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1428980 1509184 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrical stimulation to promote osteogenesis using conductive polypyrrole films
ترجمه فارسی عنوان
تحریک الکتریکی برای ترویج استئوژنز با استفاده از پلیپیررولهای رسانایی
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد بیومتریال
چکیده انگلیسی


• Polypyrrole (PPy) with adjustable resistance demonstrates good biocompatibility.
• Conductive PPy films can be deposited to surfaces with transparent appearances.
• PPy films accelerate the differentiation of rat bone marrow stromal cells (rBMSCs).
• PPy incorporated electrical devices highly improve the mineralization of rBMSCs.

In this study, we developed an electrical cell culture and monitoring device. Polypyrrole (PPy) films with different resistances were fabricated as conductive surfaces to investigate the effect of substrate-mediated electrical stimulation. The physical and chemical properties of the devices, as well as their biocompatibilities, were thoroughly evaluated. These PPy films had a dark but transparent appearance, on which the surface cells could be easily observed. After treating with the osteogenic medium, rat bone marrow stromal cells cultured on the PPy films differentiated into osteoblasts. The cells grown on the PPy films had up-regulated osteogenic markers, and an alkaline phosphatase activity assay showed that the PPy films accelerated cell differentiation. Alizarin red staining and calcium analysis suggested that the PPy films promoted osteogenesis. Finally, PPy films were subjected to a constant electric field to elucidate the effect of electrical stimulation on osteogenesis. Compared with the untreated group, electrical stimulation improved calcium deposition in the extracellular matrix. Furthermore, PPy films with lower resistances allowed larger currents to stimulate the surface cells, which resulted in higher levels of mineralization. Overall, these results indicated that this system exhibited superior electroactivity with controllable electrical resistance and that it can be coated directly to produce medical devices with a transparent appearance, which should be beneficial for research on electrical stimulation for tissue regeneration.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: C - Volume 37, 1 April 2014, Pages 28–36
نویسندگان
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